Azo-Dye Estimation of Bisphenol A Release from Plastic Bottles to Baby's Milk Via Diazotization reaction

Bisphenol A (BPA) is an organic compound commonly used in modern industries, particularly in the production of polycarbonate plastics and epoxy resins. It is classified as an endocrine-interfering compound due to its chemical structure, which is similar to that of estrogen. In this study, BPA is forced to release from plastic baby bottles to milk by heating; then it is extracted from milk and determined spectrophotometrically using an azo coupling reaction with the diazotized p-nitroaniline (PNA)reagent to yield the azo dye which shows maximum absorbance at 478 nm. The method demonstration range is from 0.1 to 5.5 µg/ml, with a limit of detection (LOD) of 0.02861 µg/ml, limit of quantitation (LOQ) 0.09538 The calculated molar absorptivity confirmed 3.2× 104 l. mol -1.cm -1. The proposed method has high sensitivity, reliability, and it is applied for determination of BPA in milk after accelerating release of BPA under the effect of heat. The results show that the plastic used in the bottles released BPA into the milk at similar levels, but the Chinese AVEHINI plastic bottle released the highest levels. The validity of the method was confirmed using standard addition and t-test.