Evaluation Level of Gene Expression for PRE gene on Preptin Hormone in Patients with Osteoporosis in Mosul City

Osteoporosis is commonly called a "silent condition" due to its tendency to progress without clear symptoms. It is defined by a reduction in bone mass, leading to weakened bones. has become more widespread, leading to sudden fractures in patients without any prior warning. It has been increasingly observed in people under the age of 35, prompting researchers to explore genetic factors. Hormones that regulate nutritional status, including Preptin, they play a critical role in bone metabolism.

Preptin is a recently identified peptide hormone, composed of 34 amino acids, secreted alongside insulin and amylin by pancreatic beta cells. Given its potential link to osteoporosis, the hormone Preptin was selected for further genetic study. Blood samples were collected in EDTA tubes for DNA extraction and in triazole tubes for RNA extraction. Gene expression of Preptin was analyzed in blood samples from osteoporosis and osteopenia patients in Mosul, Iraq, and compared to healthy individuals, due to the high incidence of osteoporosis and its genetic and hereditary associations. The study aimed to assess the genetic variation of Preptin genes in 40 blood samples from patients aged 35 to 75, who were receiving ongoing treatment at Ibn Sina Teaching and Al-Jamhory hospitals. A control group of 30 healthy individuals of similar age was also included.

Additionally, the research focused on detecting genetic mutations in the human DNA sequence, which has been registered in the International Gene Bank under number LC859842.