Effect of Organic Acids and Probiotics on Methicillin and Vancomycin-Resistant Staphylococcus aureus Isolated from Patients in the Dhi Qar Governorate

Governorate and how to use alternative treatments based on available, inexpensive, and safe materials for human use. The goal of this study was to find out how well some organic acids and probiotics kill clinical isolates of Methicillin-resistant Staphylococcus aureus MRSA and Vancomycin-resistant Staphylococcus aureus VRSA a big health problem. From 430 specimens studied, only 314 (73%) demonstrated growth, whereas 116 (27%) indicated no growth. Out of 314 (73%), 158 (50.3%) were recognized as gram-positive bacteria, whereas 50 (31.65%) were categorized as Staphylococcus aureus and the growth of gram-negative bacteria 156 (49.7%). We obtained and processed 10 patient samples using traditional microbiological procedures, followed by molecular testing for validation. We treated the isolates with four types of organic acids: acetic, lactic, citric, and oxalic acids. We also used locally isolated probiotics from sheep milk, sheep whey, and cow whey, and we also used a commercially available probiotic powder formulation consisting of three probiotic bacteria. Results demonstrated that organic acids showed much more antibacterial efficacy than probiotic therapy. Acetic acid had the most efficiency among the acids, successfully suppressing bacterial growth at reduced doses. Lactic acid had the next highest efficacy, followed by citric acid with moderate activity, and oxalic acid exhibited the lowest effectiveness. The probiotic formulation decreased bacterial numbers to a degree, although its effect was far less marked than that of the organic acids. Based on these findings, organic acids, especially acetic acid, may work well as alternatives to or in addition to common antimicrobials.