Isolation and Molecular Diagnosis of Bacteria Causing Bacteremia, and Study Their Relationship with Kidney Functions

Bacteremia is a life-threatening and a critical infection. In this study, 250 blood samples were collected from patients and directly cultured in brain-heart infusion broth and incubated aerobically, which was considered the primary culture. After observing any changes in the medium in the following day, a secondary culture was performed on basal blood agar and MacConkey agar. The colonies and cells morphologies were examined. Molecular diagnosis was then carried out based on the 16S rRNA gene, Following by nucleotide sequencing. 19 Gram-negative and 16 Gram-positive bacterial strains were identified. The isolated bacteria were as follows: Klebsiella pneumoniae (6), Shigella sonnei (5), Serratia surfactantfacie2) ), Pseudomonas aeruginosa (2), Burkholderia paludis (1), Escherichia coli (2), Enterobacter spp. (1), Staphylococcus aureus (2), Staphylococcus haemolyticus (2), Staphylococcus epidermidis (11), and Micrococcus yunnanensis (1).

Bacterial subspecies of Klebsiella pneumoniae was identified through the use of the Voges-Proskauer test and malonate consumption test. It was found that the dominant subspecies was K. pneumoniae subsp. ozaenae, with 5 strains out of 6 showing negative results for both tests. Only one strain belonged to K. pneumoniae subsp. pneumoniae, which was positive for both tests.The correlation between bacteremia and kidney function was investigated, as well as the effect of gender. The levels of urea and creatinine were measured in patients diagnosed with bacteremia and compared to healthy controls. Statistical analysis revealed neither significant differences in kidney function between patients and healthy individuals, nor significant differences between male and female patients or between healthy controls.