Genetic Effect of the Antifungal Griseofulvin in Aspergillus amstelodami

The present work aimed at investigating two genetic aspects of the antifungal Griseofulvin (GRF) in the ascomycetous fungus Aspergillus amstelodami.The first was its ability to act as a mutagen and the second was its ability to act as a photosensitizer for the DNA to be mutated by the non-mutagenic near UV(NUV). To this end, four sublethal or subinhibitory concentrations (500, 1000, 1250, 1500 g/ml) of the antifungal were tested for their ability to induce forward mutations resist to the toxic base analogue 8-azaguanine(azg) in conidia of this fungus.Within the experimental conditions specified in the present work,non of these two endpoints was fulfilled.