Direct Determination of Prednisolone by Derivative UV Spectroscopy

UV Derivative spectra were used for the direct quantitative determination of Prednisolone in absolute ethanol, the quantification was accomplished according to the integrated area under the peaks. The zero-order spectrum of Prednisolone show an absorption band at = 242nm, with molar extinction coefficient max = 13770 lit. mol-1. cm-1, the determination range was (0.36-50.46) g/ml with R2 =0.9998 and relative standard deviation RSD =1.28 %.
The determination ranges were (0.10- 72.09) g/ml, (0.36-72.09) g/ml for first and second-order derivatives respectively with R2 = 0.9999, 0.9998 and RSD = 1.53%, 2.55% respectively.
This indicate a more sensitive and accurate results as compared with the zero-order method. These methods were applied for the direct determination of the PRISOLONE tablets.